The ECNL is divided into 8 conferences across the country. FC Wisconsin competes in the Midwest Conference. For 2021-2022, the Midwest Conference includes:
- Eclipse Select (IL)
- Eclipse Select North (IL)
- Gretna Elite Academy (NB)
- FC Wisconsin (WI)
- Kansas City Athletics (MO)
- Michigan Hawks (MI)
- Michigan Hawks Black (MI)
- Minnesota Thunder Academy (MN)
- Vardar SC (MI)
- Sporting Blue Valley (KS)
- Sporting Iowa (IA)
- St. Louis Scott Gallagher - Illinois (IL)
- St. Louis Scott Gallagher - Missouri (MO)
In the Midwest Conference, every team will play every other team in the conference.
